• Nirmitee Virendra Kadake

The enzymes L-asparaginase, L-glutaminase, and L-arginase were chosen according to amino acids starvation in cancer cells and screened in halophilic and halotolerant bacteria.L–Asparaginase is a potential anti-cancer enzyme. We report production of L-Asparaginase from halotolerant bacterial isolates. Maximum L-Asparaginase production by submerged fermentation showed by R8 i.e 30 IU/min.After purification enzyme was characterized with respect to pH, temperature, different carbon source and substrate concentration. The enzyme, L-Asparaginase, shows maximum activity at an optimal temperature of 37⁰C. L-Asparaginase from G7 showed greater stability compared to R8 & R6.The production of enzymes increased as substrate concentration increased.The optimum temperature and pH for L-asparaginase and L-glutaminase activities in selected strains were similar to the physiological conditions of human body and the enzymes could tolerate NaCl up to 5% concentration.

L-Asparaginase, Halotolerant enzymes, Anticancer enzyme, Submerged fermentation.

"Study On Effect Of Different Parameters On Production Of Anticancer Enzyme L-Asparaginase By Halotolerant Bacteria.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 8, page no.d94-d118, August-2022, Available :http://www.jetir.org/papers/JETIR2208313.pdf

"Study On Effect Of Different Parameters On Production Of Anticancer Enzyme L-Asparaginase By Halotolerant Bacteria.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 8, page no. ppd94-d118, August-2022, Available at : http://www.jetir.org/papers/JETIR2208313.pdf