• AARTI NARAYANRAO WATTAMWAR
• DR.SHRINIVAS K. SARJE
• DR.N.B.GHIWARE
• SNEHAL SURYAVANSHI

The stem bark of Limonia acidissima Linn. were collected, processed and standardized as per official methods. Two extracts of Limonia acidissima Linn. stem bark (ethanol & acetone) were studied to detect the chemical compounds present, to evaluate acute oral toxicity & anti-anxiety activity. Phytochemical testing of extracts revealed presence of glycosides, flavonoids , carbohydrates etc. the total phenolic and flavonoid content of plant extracts was expressed as gallic acid equivalents and as rutin equivalents respectively. The result of acute oral toxicity studies of plant extracts as per standard references revealed median lethal dose (LD50) could be greater than 2000 mg/kg body weight in rats. Accordingly safe experimental dose was calculated as 100 and 200 mg/kg .From the acute toxicity studies as per reference standard and on the basis of literature survey for acetone and ethanol extract of Limonia acidissima Linn. stem bark maximum and minimum therapeutic experimental safe dose was found to be 200 mg/kg and 100 mg/kg respectively. The evaluation of in-vitro anti-anxiety activity in rats was done using various experimental models. Light-dark test and Elevated plus Maze test were performed for the evaluation of anti-anxiety activity in the ethanol test rats showed the increased anti-anxiety activity at a concentration of 200mg/kg and 100mg/kg compared acetone and control group.

Anti-anxiety, Limonia acidissima Linn. , elevated plus maze, light & dark

"Phytochemical investigation and Pharmacological Evaluation of Limonia acidissima Linn. stem bark extracts for antianxiety activity ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 8, page no.e147-e156, August-2022, Available :http://www.jetir.org/papers/JETIR2208416.pdf

"Phytochemical investigation and Pharmacological Evaluation of Limonia acidissima Linn. stem bark extracts for antianxiety activity ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 8, page no. ppe147-e156, August-2022, Available at : http://www.jetir.org/papers/JETIR2208416.pdf