• ASHISH RAJENDRA CHAUDHARI

New, simple and sensitive RP-HPLC method was developed for simultaneous estimation of Bisoprolol Fumarate and Telmisartan in bulk and tablet formulation. The present study was undertaken to develop and validate an accurate, robust, precise and cost – effective RP-HPLC method for the simultaneous estimation of Bisoprolol fumarate and Telmisartan in bulk and tablet pharmaceutical formulation. Separation was achieved on C8 Column (150mmx4.6mm,5µm) using Ammonium formate, Acetonitrile :Methanol (50:50 v/v) as a mobile phase with flow rate of 1.0ml/min. The detection carried out at wavelength 231 nm. The retention times of Bisoprolol fumarate and Telmisartan were found that 6.7 min and 9.0 min respectively. The method was validated in terms of linearity, precision, Robustness, calibration as per ICH guidelines. linearity was carried out by using concentration range 5-25 µg.mL-1 for Bisoprolol fumarate and 40-200 µg.mL-1 for Telmisartan. The method shows linearity in the mentioned concentration having correlation coefficient R2 0.999 and 0.999respectively. The mean recoveries of bisoprolol fumarate and telmisartan were found to be 99-101% respectively. The %RSD of the Bisoprolol fumarate and Telmisartan were found to be 0.38-0.54 and 0.01-0.51 respectively. The development RP-HPLC method was found to be highly specific. The method could be applicable for routine determination in bulk drug as well as dosage formulation containing Bisoprolol and Telmisartan combination.

KEY WORDS : RP-HPLC; Bisoprolol fumarate; Telmisartan; Validation; Simultaneous estimation; etc

"DEVELOPMENT AND VALIDATION OF RP -HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BISOPROLOL FUMARATE AND TELMISARTAN IN BULK AND PHARMACEUTICAL FORMULATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 8, page no.e766-e774, August-2022, Available :http://www.jetir.org/papers/JETIR2208494.pdf

"DEVELOPMENT AND VALIDATION OF RP -HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BISOPROLOL FUMARATE AND TELMISARTAN IN BULK AND PHARMACEUTICAL FORMULATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 8, page no. ppe766-e774, August-2022, Available at : http://www.jetir.org/papers/JETIR2208494.pdf