• Pradeep Swarnkar
• Mahesh Kumar Gupta
• Monika Maheshwari

Alogliptin and Metformin are used as combined medication to control high blood sugar in type-2 diabetes patient. A simple, rapid, accurate, precise UV spectrophotometric method for the simultaneous estimation of Alogliptin Benzoate and Metformin Hydrochloride in a combined tablet dosage form using the simultaneous equation method has been developed. The method is based on the simultaneous equations for analysis of both the drugs using methanol as a solvent. Metformin Hydrochloride has absorbance maxima at 231 nm and Alogliptin Benzoate has absorbance maxima at 276 nm in methanol. The linearity was obtained in the concentration range of 5–25 μg/ml and 5–25 μg/ml for Alogliptin Benzoate and Metformin Hydrochloride, respectively. The concentrations of the drugs were determined by using the simultaneous equations method. The mean recovery was 99.89 ± 1.76 and 99.79 ± 0.71 for Alogliptin Benzoate and Metformin Hydrochloride, respectively. The method was found to be simple, accurate, and precise and was applicable for the simultaneous determination of Alogliptin Benzoate and Metformin Hydrochloride in the pharmaceutical tablet dosage form. The results of analysis have been validated statistically and by recovery studies. It is a cost efficient and time saving method which has many applications in stability studies, forced degradation studies, routine analysis, quantification, etc. It can be successfully acquired for daily quality control analysis of alogliptin and metformin in dosage form.

Alogliptin Benzoate, Metformin Hydrochloride, simultaneous equation, UV spectroscopy, analysis

"METHOD DEVELOPMENT FOR THE SIMULTANEOUS ANALYSIS OF METFORMIN AND ALOGLIPTIN BY USING UV-VISIBLE SPECTROPHOTOMETER", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 9, page no.e756-e761, September-2022, Available :http://www.jetir.org/papers/JETIR2209490.pdf

"METHOD DEVELOPMENT FOR THE SIMULTANEOUS ANALYSIS OF METFORMIN AND ALOGLIPTIN BY USING UV-VISIBLE SPECTROPHOTOMETER", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 9, page no. ppe756-e761, September-2022, Available at : http://www.jetir.org/papers/JETIR2209490.pdf