• Velisetty Tharaka Rama
• Sathish Thadikamala
• Sudhakar P
• Krishna Satya Alapati

Recently WHO listed L-asparaginase as one of the essential medicines for the treatment of acute lymphoblastic leukemia in children. Due to the increase in the usage of this enzyme in the treatment of leukemia and other industries such as food and biosensors, it is gaining more attention by many industries. To meet the demand of the industry’s requirement it is necessary to increase the yield as well as to discover new strains which can produce higher titers of this enzyme. In the present study high yielding bacterial strain was isolated from the Krishna River soils. The isolated bacteria were identified as Bacillus subtilis by both biochemical and molecular methods. The strain was designated Bacillus subtilis THARAKA. Various media were screened for a higher amount of L-asparaginase production by isolated B.subtilis THARAKA. Along with media, various fermentation parameters were optimized by one factor at a time method. Overall, by optimization of various fermentation parameters, 1.8 folds of L-asparaginase yield was enhanced.

L-Asparaginase, L-Asparagine, Acute lymphocytic leukemia, Acrylamide Isolation, Bacillus subtilis

"Isolation, Identification of Anti-Leukemic Agent L-Asparaginase Producing Bacteria From Krishna River Soils and Enhancement of Enzyme Production", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 10, page no.b419-b450, October-2022, Available :http://www.jetir.org/papers/JETIR2210164.pdf

"Isolation, Identification of Anti-Leukemic Agent L-Asparaginase Producing Bacteria From Krishna River Soils and Enhancement of Enzyme Production", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 10, page no. ppb419-b450, October-2022, Available at : http://www.jetir.org/papers/JETIR2210164.pdf