• Manita Ahlawat
• Prof. Pawankumar Godatwar

Introduction : A wide spectrum of infectious diseases are emerging with multi drug resistant pathogens. With the rising trends of antimicrobial resistance, the researchers are in constant search of new herbal agents with immense antimicrobial potential. Shigru one of the drugs indicated in krimighan mahakashaya by Acharya Charaka has been selected for the study to prove its antibacterial efficacy on scientific grounds. Objective To evaluate the antibacterial effect of aqueous and ethanolic extracts of Moringa oleifera Lam. Stems Bark on the growth of gram-positive and negative bacteria. Methods Aqueous and ethanolic extracts of Shigru with three concentrations 10%, 20%, 30% were prepared using Continuous extraction method by Soxhlet Apparatus. All extracts were tested against Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 27853). The susceptibility tests were performed using Agar ditch method. The antibacterial activity was assessed on the basis of Zone of inhibition and activity index. Results Aqueous extract and ethanolic extract of Shigru showed mean ZOI (in mm) 22.33, 27.33, 28.33 and 11.67, 14.3, and 23.33mm at 10%, 20 % and 30 % concentration respectively against S.aureus. In case of E.coli, at 10%, 20%, and 30% concentrations, the average ZOI (in mm) for Shigru was recorded as 0, 0 and 14.67 with aqueous extract and 0, 12.67 and 23.67 with ethanolic extract respectively as compared to 27.33 and 26.67 mm ZOI in the corresponding positive controls. The average ZOI (in mm) was reported as 13.33, 18 and 22.33 with aqueous extract and 0, 13.67 and 23.33 with ethanolic extract of Shigru at 10%, 20%, and 30% concentrations respectively as compared to 25.33 and 26.33 mm ZOI in the positive control against P. aeruginosa. Conclusion: For both aqueous and ethanolic extracts of Shigru, the inhibitory effect was maximum in S.aureus followed by P.aeruginosa and least in E.Coli. The study indicates that Shigru possess a significant antimicrobial potential and may be used as alternate treatment for infections caused by tested strains.

Key words : Shigru, Antibacterial Activity, Zone Of Inhibition, Activity Index.

"Evaluation of In Vitro Antibacterial Activity of various extracts of Shigru", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 10, page no.c578-c587, October-2022, Available :http://www.jetir.org/papers/JETIR2210274.pdf

"Evaluation of In Vitro Antibacterial Activity of various extracts of Shigru", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 10, page no. ppc578-c587, October-2022, Available at : http://www.jetir.org/papers/JETIR2210274.pdf