• P. Sreenivasula Reddy
• G. Rupendra

The present study was aimed to investigate individual and combined effect of cafeteria diet and alcohol for reproductive toxicities in male Wistar rats. A total of twenty-four rats were divided into four groups. Animals in the group 1 were maintained on normal diet and served as controls, and animals in group 2 were maintained on cafeteria (CAF) diet. Rats in the 3rd group were administered with alcohol (2.0 g/kg/bw/day) and animals in the 4th group were maintained on cafeteria diet and also administered with alcohol for 60 days. Compared with controls, rats maintained on CAF diet showed increased body weight gain. Further, increased body weight gain was observed in rats co-administered with CAF and alcohol compared to control rats. Conversely, alcohol treated rats did not show any significant change in body weight compared to control. The relative weights of testes, cauda epididymis, seminal vesicles and prostate glands were significantly decreased in alcohol alone or combined cafeteria diet exposure rats compared to control rats (P < 0.05). Daily sperm production, numbers of motile-, viable-, and HOS-tail swelled sperm decreased significantly in all experimental rats. Circulatory levels of testosterone and testicular steroidogenic marker enzyme activities (3 β-HSD, 17 β-HSD) were significantly reduced in all experimental rats. The results also displayed significant decrease of antioxidant enzyme (SOD, CAT, GPx, and GR) activities and remarkable depletion in MDA and reduced glutathione content in testes of all experimental rats. In summary, these results indicated that CAF diet combined with alcohol intake suppresses spermatogenesis and testicular steroidogeneses and altered tissue oxidative stress there by resulting in reproductive toxicity. Further, deterioration in spermatogenesis and steroidogeneses was observed in rats maintained of HFD and treated with alcohol than individual treatments. Thus, CAF diet and alcohol has synergetic effect on male reproductive system.

Alcohol; Obesity; Reproductive Toxicity; Cafeteria diet

"Reproductive toxicological effects of cafeteria diet, alcohol and their combination on sperm quality parameters, steroidogenic enzyme activities, testicular antioxidant capacity in male Wistar rats", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 1, page no.d516-d521, January-2023, Available :http://www.jetir.org/papers/JETIR2301365.pdf

"Reproductive toxicological effects of cafeteria diet, alcohol and their combination on sperm quality parameters, steroidogenic enzyme activities, testicular antioxidant capacity in male Wistar rats", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 1, page no. ppd516-d521, January-2023, Available at : http://www.jetir.org/papers/JETIR2301365.pdf