• Rashmi Pujar
• Nivedita Pujari
• Arun K Shettar
• Joy H Hoskeri
• Ramesh Babu Y

Abstract Cerium oxide nanoparticles are potential nanosized compounds established to have several biomedical applications. Croton sparsiflorus is a wild shrub considerd to have various medicinal applications. In current study green synthesis of cerium oxide nanoparticles is carried out from Croton sparsiflorus extract. CeO-NPs are characterized by UV-visible spectroscopy, SEM, EDX and particle size analysis. UV-visible spectroscopy analysis reported presence of cerium oxide nanoparticles, which was reported by change in colour to brown and with a λmax at 330nm. SEM analysis showed presence of round and spherical nanoparticles of 20nm. Particle size analysis carried out using Dynamic light scattering reported an average diameter of 126.2nm of nanoparticles. EDX analysis confirmed the elemental composition of nanoparticles with presence of oxygen and cerium. Cerium oxide nanoparticles were also investigated for antioxidant activity, antimicrobial and genoprotective activity. Antioxidant activity was carried using DPPH assay, FRAP assay, H2O2 and Phosphomolbdenum assay. FRAP assay showed highest antioxidant activity with IC50 value 533.78µg/ml. CeO-NPs showed antimicrobial activity against gram positive bacteria Bacillus cerus with zone of inhibition of 9mm and 10mm. Therefore cerium oxide nanoparticles synthesized from Croton sparsiflorus extract are thus potential compounds that can be used in various biomedical applications.

Key words: Cerium oxide, nanoparticles, Croton sparsiflorus, antibacterial and antioxidant.

"Green synthesis, characterization and evaluation of biological activity of cerium oxide nano-particles synthesized from Croton sparsiflorus", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 2, page no.f394-f407, February-2023, Available :http://www.jetir.org/papers/JETIR2302547.pdf

"Green synthesis, characterization and evaluation of biological activity of cerium oxide nano-particles synthesized from Croton sparsiflorus", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 2, page no. ppf394-f407, February-2023, Available at : http://www.jetir.org/papers/JETIR2302547.pdf