• Yuganti N. Belsare
• Shailesh G. Jawarkar
• Piyusha Gulhane
• Snehaja Bhalerao
• Saurabh Ingle

Scientific researchers' interest in noninvasive sample techniques for illness diagnosis and therapeutic medication monitoring has grown over the past few years. Dried saliva spot (DSS) was employed as a saliva collection procedure for the first time. Using an organic solvent, 50 μL of saliva were extracted using the DSS process after being collected on filter paper. Liquid chromatography and mass spectrometry were employed in the DSS to identify the local anaesthetic lidocaine as a model substance. This has led to the widespread use of dry saliva spot (DSS), a sampling method for gathering dried saliva samples, as an alternate matrix to serum for the detection of target molecules. The effectiveness of the preparation and analysis of biological samples is increased when the DSS method is used in conjunction with a highly sensitive detection instrument. More topics related to the DSS method, such as dried blood spots, dried plasma spots, and dried matrix spots, are covered. Saliva offers a benefit over other biological fluids used in dried spot procedures, such as serum, tears, urine, and plasma, in terms of sample collection from young children or people with disabilities. By demonstrating several dried spot methods, this study seeks to offer essential strategies and principles for dried spot methods to investigate biological materials. In this article, we review current developments in DSS techniques from June 2014 to March 2021 and talk about the benefits and drawbacks of the main components of this technique, such as sample preparation and method validation. Finally, we discuss the difficulties and potential of these techniques in real-world settings.

Keywords: Human saliva, Dried saliva spot, Therapeutic drug monitoring, Disease diagnosis

"A REVIEW :THE USE OF DRIED SALIVA SPOTS (DSS) FOR THE POSSIBLE MONITORING OF TREATMENT DRUGS AND THE DIAGNOSIS OF DISEASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 3, page no.c194-c207, March-2023, Available :http://www.jetir.org/papers/JETIR2303227.pdf

"A REVIEW :THE USE OF DRIED SALIVA SPOTS (DSS) FOR THE POSSIBLE MONITORING OF TREATMENT DRUGS AND THE DIAGNOSIS OF DISEASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 3, page no. ppc194-c207, March-2023, Available at : http://www.jetir.org/papers/JETIR2303227.pdf