• Ayesha Asghar
• Maira Iftikhar
• Muhammad Bilal Arif

Background: Stress becomes the cause of the disruption in the functioning of the body’s systems. Overall health and well-being only depend on the functioning of the microbiome such as; digestion, the situation of equilibrium, vitamin level synthesis, and regulation of the functioning of neurotransmitters that eventually affect the immune system of the individual. Aim of the study: The aim of the study is to investigate the role of the gut microbiome in the bidirectional relationship between stress and inflammation. Methodology: The randomized Control Trial (RCT) has been conducted on a sample of N=30 participants recruited from the general population. The participants were randomly assigned to the experimental group and control group. The experimental group was involved in taking dietary intervention for the 7 weeks so stress reduction would not be enhanced or for the healthy gut. The initial screening of the participants was done on the basis of inclusion and exclusion criteria such as participants aged 18 to 65 years, willing to participate in the study, and not having any chronic illness. The Trier Social Stress Test (TSST) was used for measuring the stress level among individuals of both groups. Furthermore, Immunohistochemistry and HPLC were used to measure the level of neurotransmitters. Ethical consideration has been followed throughout the study. Results: the results of the study indicated that there is evidence that the gut microbiome may play a role in modulating the inflammatory response to stress. Furthermore, the findings highlight the importance of considering the bidirectional relationship between stress and inflammation in the context of health and disease.

Stress, Gut Microbiome, Inflammation, dietary aspect, neurotransmitter

"The Role of the Gut Microbiome in the Bidirectional Relationship between Stress and Inflammation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 4, page no.k403-k413, April-2023, Available :http://www.jetir.org/papers/JETIR2304A58.pdf

"The Role of the Gut Microbiome in the Bidirectional Relationship between Stress and Inflammation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 4, page no. ppk403-k413, April-2023, Available at : http://www.jetir.org/papers/JETIR2304A58.pdf