• Smita S. Kharkale-Bhuyar

Study investigated effect on Allium sativum L. (garlic) and Allium cepa L. (onion) by organic substance of 1,2,4,5-tetrazin were analyzing root chromosomal abnormalities. The root tips of the two plant species were treated with different concentrations (0.001M, 0.002M, 0.003M) of 1,2,4,5-tetrazin mixed with 10% Dimethyl Sulfoxide-Water. After treatment, various types of physiological hydrolyze, squashes, mitotic index (MI), cytological abnormalities, and chromosomal aberrations were scored. The results indicated a genotoxic effect, with a significant reduction in the mitotic index found to be dose-dependent. The findings suggest that 1,2,4,5-tetrazin has a toxic effect on the root cells of garlic and onion, and that the magnitude of the effect increases with increasing concentration of the chemical.

Keywords: Mitotic index, genetoxicity, root-shoot ratio, Chromosomal aberrations.

"Effect on Allium cepa and Allium sativum by organic substance of 1,2,4,5-tetrazin", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 4, page no.k445-k452, April-2023, Available :http://www.jetir.org/papers/JETIR2304A62.pdf

"Effect on Allium cepa and Allium sativum by organic substance of 1,2,4,5-tetrazin", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 4, page no. ppk445-k452, April-2023, Available at : http://www.jetir.org/papers/JETIR2304A62.pdf