• GOVIND ETHAPE
• DR. ITISHREE VAIDYA

This work reports a simple scheme for extracting plant based biocatalyst from cost effective source and then transform the pharmaceutical compound by utilizing this biocatalyst. Crude lipase extracted from Arachis hypogaea seed by liquid extraction method. Then protein content of Arachis hypogaea crude lipase extract was estimated by using Folin Lowry method. Enzymatic activity of crude lipase extract was estimated by the enzymatic hydrolysis of variety of oils namely as sunflower oil, coconut oil, soyabean oil, olive oil. The crude seed lipase extract activity is compared with commercially available fungal lipase. Comparably the highest seed lipase activity was found to be 0.326 (U/ml) for hydrolysis of coconut oil. Arachis hypogae seed crude lipase also used for hydrolysis of p-nitrophenyl acetate substrate and liberated p-nitrophenol and it is determined by UV Visible spectroscopy at λmax 400 nm and after 180 min of reaction completed it showed 88 % consumption of p-nitrophenyl acetate.

EXTRACTION AND ENZYMATIC ACTIVITY STUDIES FOR ARACHIS HYPOGAEA LIPASE

"EXTRACTION AND ENZYMATIC ACTIVITY STUDIES FOR ARACHIS HYPOGAEA LIPASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 5, page no.h587-h599, May-2023, Available :http://www.jetir.org/papers/JETIR2305773.pdf

"EXTRACTION AND ENZYMATIC ACTIVITY STUDIES FOR ARACHIS HYPOGAEA LIPASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 5, page no. pph587-h599, May-2023, Available at : http://www.jetir.org/papers/JETIR2305773.pdf