• Abhay Kumar Bansal
• Ramasamy Rathinam
• Suraj Choudhary

Abstract: In this study, pentafluorophenol was coupled to create (S)-naproxen-based active chiral derivatizing reagents (CDR), which were then synthesized and characterized using UV, IR, HRMS, NMR, and CHNS. In this work racemic propranolol (-blocker) was chosen for the chiral recognition study. Highly reactive CDR was used to create the diastereomers of the selected -blocker under microwave heating conditions. Highly reactive CDR was used to create the diastereomers of the selected -blocker under microwave heating conditions. , RP-HPLC was employed to separate the produced diastereomers. The diastereomers and chromatographic conditions were studied by altering the mobile phase and sample concentrations. The synthesized diastereomer’s elution order was predicted using the optimized structures. The optimized structures were used to predict the elution order of diastereomers synthesized. LOD (0.192 ng mL−1), LOQ (0.576 ng mL−1), calibration range (0.01-1.0 mg mL−1), correlation coefficient, and recovery were the validation parameters for our method (99.54 percent for intra-day assay and 99.89 percent for inter-day assay).

"Derivatization of racemic propranolol by naproxen based chiral reagent and separation on RP-HPLC, and structural study using DFT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 5, page no.n289-n296, May-2023, Available :http://www.jetir.org/papers/JETIR2305D43.pdf

"Derivatization of racemic propranolol by naproxen based chiral reagent and separation on RP-HPLC, and structural study using DFT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 5, page no. ppn289-n296, May-2023, Available at : http://www.jetir.org/papers/JETIR2305D43.pdf