• Indu Mittal
• Dr.K.Sarvanan
• Dr.Amarjeet Singh

Abstract- This study evaluated the cytotoxic and anti-inflammatory properties of an Emulgel formulated using the leaves of Nyctanthes arbor-tristis. The study found that the Emulgel had no cytotoxic effect on RAW 264.7 cell lines at concentrations of 5mg/mL. The anti-inflammatory properties of the Emulgel were assessed by measuring the release of IL-6 from the treated cells, with Formulation 1 showing the potential as an anti-inflammatory agent. The Emulgel was evaluated for its organoleptic properties, homogeneity, pH determination, spreadability, viscosity, extrudability, in vitro drug release, and stability studies. The phytochemical screening confirmed the presence of alkaloids, carbohydrates, saponins, glycosides, steroids, phenols, and flavonoids in the extract of the leaves. The evaluation parameters for the Emulgel showed that Formulation 2 consistently performed well and was the best among other formulations. The stability studies indicated that Formulation 2 was stable and maintained its physical appearance, spreadability, pH, and drug content parameters over a 3-month period at various temperatures. Future research may involve in vivo testing of the formulation in an osteoarthritis model.

Keywords - Nyctanthes Arbor Tristis Linn Emulgel, Anti-Inflammatory Emulgel, Emulgel, Anti-Osteoarthritic Emulgel

"A Research on In Vitro Investigation of the Anti-Osteoarthritic and Anti-Inflammatory Properties of Nyctanthes Arbor Tristis Linn Emulgel", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 7, page no.e617-e631, July-2023, Available :http://www.jetir.org/papers/JETIR2307466.pdf

"A Research on In Vitro Investigation of the Anti-Osteoarthritic and Anti-Inflammatory Properties of Nyctanthes Arbor Tristis Linn Emulgel", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 7, page no. ppe617-e631, July-2023, Available at : http://www.jetir.org/papers/JETIR2307466.pdf