UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 10 Issue 8
August-2023
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2308056


Registration ID:
522698

Page Number

a435-a440

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Title

Confirming Hybridity in Grape (Vitis vinifera L) using Microsatellites

Authors

Abstract

The microsatellites markers used in genetic analysis does not only allow differentiation but also identification and parentage analysis of grapevine cultivars. The main purpose of this study was to confirm the hybridity of these accessions and to construct a molecular database including the parents commonly grown in India. A total of eighteen hybrids and seven parents were analyzed using twelve microsatellite simple sequence repeat (SSR) markers. The hybrid nature of all the progenies used was marked by VVMD-32. In VVMD-32, unique banding pattern was observed in Black Champa (male parent) in Group A. In which six bands were prominent, out of which first and last band were absent in Thompson Seedless (female parent). While in case of primer VVS-29, three prominent bands were observed in male parents. This primer was able to confirm the hybridity of progenies of group A, F, G and H. The primer VVS-2 confirmed the hybridity of progenies of group-B, C and H. In case group B, the 3rd band of male parent was distinct from the female parent. These three primers were thus able to confirm the hybrid nature of all the progenies. The use of twelve polymorphic microsatellite markers to detect the hybrids within Indian grapevine germplasm suggested that this is a reliable, efficient, and effective marker system that can be used for parentage studies in grapevine and subsequently in crop improvement programs

Key Words

hybridity, microsatellites markers, Vitis vinifera L.

Cite This Article

"Confirming Hybridity in Grape (Vitis vinifera L) using Microsatellites", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 8, page no.a435-a440, August-2023, Available :http://www.jetir.org/papers/JETIR2308056.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Confirming Hybridity in Grape (Vitis vinifera L) using Microsatellites", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 8, page no. ppa435-a440, August-2023, Available at : http://www.jetir.org/papers/JETIR2308056.pdf

Publication Details

Published Paper ID: JETIR2308056
Registration ID: 522698
Published In: Volume 10 | Issue 8 | Year August-2023
DOI (Digital Object Identifier):
Page No: a435-a440
Country: Bengaluru, karnataka, India .
Area: Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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