• Miss.Pallavi V Ganvir
• Rupesh V Ingole
• Dr. S R Butle
• Mohd Ameen Mahmood
• Mohammad Zishan Ibrahim

In present study. Phytochemical investigation and in-vitro anticancer activity of Aegle marmelos Linn. was performed. Standardization of powder is done by determination of ash value, extractive value and moisture content of bark and leaf of Aegle marmelos Linn Thin layer chromatography was performed for the separation of active constituents from ethyl acetate, chloroform, methanol and aqueous extract. Methanol fractions of leaf & bark and aqueous extract of leaf of Aegle marmelos L leaf and bark were tested for in-vitro anticancer activity. In-vitro anticancer activity was carried out by using SSC 40 oral cancer cell line. Characterization of methanolic extract of bark and leaf was carried out by using NMR (nuclear magnetic resonance) spectroscopy and methanolic extract of leaf by using HR-LCMS (High resolution- liquid chromatography mass spectroscopy) analysis. The obtained ash value is in the range of 12.8% w/w and the yield of leaf extract was found to be in the range of 5.78% and the yield of bark extract was found to be in the range of 6.04. RF value was found to be 0.91. Phytochemical screening confirmed the presence of alkaloids, glycosides, carbohydrates, flavonoids, phenol amino acids and proteins. At any concentration SSC 40 cell line didn't shows inhibition of cancer cells, observed by calculating LC50, TGI and G150. At 20 ug/ml concentration, aqueous extract shows more cell inhibition in oral cancer cell line than methanolic leaf extract and methanolic bark extract. Phytochemical investigations of methanol extract were performed by NMR and HR-LCMS analysis. Result indicates that methanolic extract of leaf and bark and aqueous extract of leaf of Aegle marmelos L having significant anticancer activity and various compounds are identified by using HR-LCMS analysis.

Keywords; Cancers, Aegle Marmelos Linn, Anti-cancer activity, Oral cancer.

"In-vitro Anticancer Activity of Aegle Marmelos Linn", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 9, page no.a787-a793, September-2023, Available :http://www.jetir.org/papers/JETIR2309094.pdf

"In-vitro Anticancer Activity of Aegle Marmelos Linn", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 9, page no. ppa787-a793, September-2023, Available at : http://www.jetir.org/papers/JETIR2309094.pdf