• Dr.Y.Vijaya Bharathi
• Dr.K.Jwalamukhi

Two Black gram varieties LBG-806 (tolerant variety) and LBG-823 (susceptible variety) were selected for comparative analysis of molecular responses at different temperature treatments (as per TIR protocol). In T.I.R technique, during induction treatment plants acquire thermo-tolerance through several mechanisms. Both the selected cultivars showed varied banding pattern with RAPD-PCR amplification at different temperature treatments. Results of RAPD-PCR amplification, in susceptible variety upon induction treatment showed three extra bands i.e., for OPC-5 primer, a band below 100bp, a band at 200bp, a band between 400-500bp sizes. Presence of high intensity bands and extra bands in both varieties in induction treatment indicated that they have been acclimatized and hardened at the time of induction temperature. It is concluded that this marker assisted analysis provided supportive molecular information for relevance of TIR based screening technique.

TIR, Black gram, SDS-PAGE, RAPD-PCR.

"Assessment of Genetic Variability for Acquired Thermo-Tolerance in Two Black gram Varieties Using RAPD Markers", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 9, page no.f32-f40, September-2023, Available :http://www.jetir.org/papers/JETIR2309504.pdf

"Assessment of Genetic Variability for Acquired Thermo-Tolerance in Two Black gram Varieties Using RAPD Markers", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 9, page no. ppf32-f40, September-2023, Available at : http://www.jetir.org/papers/JETIR2309504.pdf