• Sanjeevakumaar
• Blessy Jacob
• D. Visagaperumal
• Vineeth Chandy

The study was aimed to isolate, phytochemical analysis, biological screening and in-silico antidiabetic activity of Oldenlandia auricularia. From the present work the chemical compounds from leaves of Oldenlandia auricularia has been extracted successfully. Methanol, ethanol and chloroform are used individually for extraction and to find extractive value. The higher extractive value is found in the chloroform solvent. This research work visualized successfully the application of column chromatography techniques for the isolation of biologically active secondary metabolites from the plant sample. After separating the fractions using the column chromatography, all the fractions were checked, using the TLC, and as well as subjected to FT-IR and Mass spectroscopy for the identification of compounds. The screening of antidiabetic activity was carried out by in-vitro method. In-vitro antidiabetic activity was carried out, which showed that more activity was present in chloroform extract of Oldenlandia auricularia. In-silico antidiabetic activity was also carried out by using software like Autodock and found the good binding affinity from the component present in the Oldenlandia auricularia leaves. Molecular docking was taken as first step in this research as it is a powerful tool for the ligand-based drug discovery. The investigation concludes that the ligand (Auricularine) was docked with the target alpha-amylase inhibitor (44w93 receptor) and, compared with the standard (Acarbose with 4w93) and also studied their ADMET properties by using SwissADME software.

Oldenlandia auricularia, Antidiabetic activity, In-silico, In-vitro, Alpha-amylase, Chemical constituents, Plant extract, Chloroform, Methanol, Auricularine, Spectroscopy, Ligand, Receptor, 4w93.

"Isolation, Phytochemical Investigation and Biological Screening in leaves of Oldenlandia auricularia", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 10, page no.a745-a754, October-2023, Available :http://www.jetir.org/papers/JETIR2310083.pdf

"Isolation, Phytochemical Investigation and Biological Screening in leaves of Oldenlandia auricularia", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 10, page no. ppa745-a754, October-2023, Available at : http://www.jetir.org/papers/JETIR2310083.pdf