• Dr Shivakumara K N

Abstract : In the pursuit of more biologically potent compounds, we envisioned to synthesize a series of N1-alkyl/aryl-N5-(6-nitro-1,3-benzothiazol-2-yl)-1,5-thiobiurets by reacting 6-nitro-2-aminobenzothiazole with phenylchloroformate using anhydrous pyridine in dry THF at room temperature (RT) to initially produce corresponding isocyanates. The resulting isocyanate is then refluxed for 10-12hours with monoalkyl/aryl urea derivatives in the presence of sodium hydride (NaH) in dry THF. The synthesized biuret compounds were identified using 1H NMR and Rf values, and subsequently tested for antibacterial activity against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, as well as antifungal activity against Aspergillus niger, Aspergillus flavus, and Fusarium moniliforme. Some of the biurets displayed significant activity, while others showed moderate activity.

Index Terms - Antimicrobial resistance, thiobiuret, 6-nitro-2-aminobenzothiazole, zone of inhibition, isocyanates and Tetrahydrofuran (THF).

" SYNTHESIS AND DEVELOPMENT OF N1-ALKYL/ARYL-N5-(6-NITRO-1, 3-BENZOTHIAZOL-2-YL)-1, 5-THIOBIURETS AND THEIR ASSESSMENT FOR ANTIMICROBIAL EFFICACY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 11, page no.f668-f675, November-2023, Available :http://www.jetir.org/papers/JETIR2311580.pdf

" SYNTHESIS AND DEVELOPMENT OF N1-ALKYL/ARYL-N5-(6-NITRO-1, 3-BENZOTHIAZOL-2-YL)-1, 5-THIOBIURETS AND THEIR ASSESSMENT FOR ANTIMICROBIAL EFFICACY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 11, page no. ppf668-f675, November-2023, Available at : http://www.jetir.org/papers/JETIR2311580.pdf