• Dr. Sushil Kumar

This study investigated the potential of plant tissue culture for the sustainable production of secondary metabolites from Cassia fistula L. (Indian Laburnum). Callus cultures were established and optimized using a combination of plant growth regulators (PGRs) to maximize the production of phenolics, which are the known active ingredients with medicinal properties. The experiment identified contrasting effects between individual auxins (NAA, 2,4,5-T, IBA, IAA) and their interactions with cytokinins (Kn, BAP) on callus metabolism. NAA generally promoted both carbohydrate and phenolic accumulation, while IBA specifically favored phenolics. IAA, at lower concentrations, promoted sugar synthesis but potentially induced stress at higher levels. The type and concentration of cytokinin further influenced these responses. Combinations of BAP with NAA or IBA showed promise for balancing carbohydrate and phenolic production, whereas Kn often led to stress responses with variable effects on phenolics. The study revealed that the highest total phenolic content was achieved in calli derived from media supplemented with 2.0 mg/L of the synthetic auxin 2,4,5-T. However, this treatment resulted in poorer callus growth. Conversely, callus with superior growth attributes exhibited lower phenolic content. These findings suggest a trade-off between callus growth and targeted metabolite production, highlighting the importance of optimizing PGR combinations for the desired outcome. In conclusion, this research demonstrates the feasibility of utilizing plant tissue culture for the sustainable production of phenolics from C. fistula. The study emphasizes the importance of selecting appropriate PGR combinations to achieve a balance between callus growth and the targeted production of medicinally valuable secondary metabolites.

Medicinal plants, secondary metabolites, growth index, fresh weight, dry weight, plant growth regulators

"Optimizing Plant Tissue Culture of Cassia fistula L. for Enhanced Phenolic Production through Auxin and Cytokinin Modulation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 12, page no.h463-h469, December-2023, Available :http://www.jetir.org/papers/JETIR2312758.pdf

"Optimizing Plant Tissue Culture of Cassia fistula L. for Enhanced Phenolic Production through Auxin and Cytokinin Modulation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 12, page no. pph463-h469, December-2023, Available at : http://www.jetir.org/papers/JETIR2312758.pdf