• ANJU AJIKUMAR
• Dr. Keerthi T.R
• Nithin T.U

Abstract :The isolation, molecular identification and antimicrobial potential of marine bacteria Alcanivorax xenomutant was performed. The 16SrDNA sequences were deposited in GenBank with accession number MN103744. The bioactive profiling of the strain was done for the first time. The extraction of bioactive compounds was performed using ethyl acetate. The antimicrobial properties were investigated against Klebsiella pneumonia (MTCC 432), Salmonella typhi (MTCC 733), Bacillus subtilis (MTCC 441), E.coli (MTCC 443) and Staphylococcus aureus (MTCC 1430). The results showed that the ethyl acetate extract showed higher and broad spectrum efficacy against all the tested organisms in comparison with the erythromycin standard. The purification of the bioactive compounds was done by thin layer chromatography using Chloroform:Methanol as a solvent system. The characterisation of compounds was done by GC- MS analysis and the identified compounds are 2H-1,3-Benzoxazine, octahydro-2-3-nitrophenyl, 2,4-Di-tert-butylphenol, Ethyl homovanillate and Quinoline, 1,2-dihydro-2,2,4-trimethyl. These bioactive compounds were docked against the enzymes such as tyrosyl-tRNA syntehtase and DNA gyrase with the help of Autodock 4. Docking studies were carried out for all the ligands; the compounds binding to the active site of the enzymes and the binding energy was compared with that of the Erythromycin standard. The molecular docking study confirms the antimicrobial activity of ethyl acetate extract of marine bacteria Alcanivorax xenomutant.

Index Terms : Marine bacteria; Bioactive compound; Antimicrobial activity; Antioxidant activity; TLC; GCMS; Molecular docking.

"Antimicrobial property and insilico molecular docking studies of bioactive compounds from Alcanivorax xenomutant MN103744", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 1, page no.d32-d43, January-2024, Available :http://www.jetir.org/papers/JETIR2401307.pdf

"Antimicrobial property and insilico molecular docking studies of bioactive compounds from Alcanivorax xenomutant MN103744", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 1, page no. ppd32-d43, January-2024, Available at : http://www.jetir.org/papers/JETIR2401307.pdf