• Shubhas Choudhari
• Jagdish Chandra Rathi
• Usha Vishwakarma

Wound healing is a typical biological process. It involves four intricate steps: homeostasis/coagulation; inflammation, migration, and proliferation; reepithelialization; and restoration. A number of mediators, including platelets and cytokines, inflammatory cells, cellular and extracellular matrix, proteinases, growth factors, and inhibitors, have an impact on each stage of the healing process for wounds. In the present work, a polyherbal gel formulation for wound healing activity was developed using methanolic extract of Vaccinium macrocarpon and Cissampelos pareira. The developed formulation was evaluated for organoleptic parameters like colour, odour and physicochemical parameters like pH, viscosity, spreadability, skin irritation study, etc. In-vitro antibacterial activity of both the extracts carried out against Escherichia coli by well diffusion assay. Apart from that, the wound healing activity of a polyherbal formulation was evaluated by an incision model on wistar rats. The results showed that the polyherbal formulation exhibited slightly yellowish grey colour with a smooth texture and Semisolid gel. The gel showed pH ranged from 6.1- 6.5, viscosity was F1-1453±0.70, F2-1436±0.27 and F3-1489±0.83cps with spindle number 63, and spreadability was found to be F1-23.99, F2-24.19 and F3-25.20 (g.cm/s), and no irritation was found on the healthy volunteer’s skin. In the wound healing activity, polyherbal did not affect the normal feed and water uptake. The wound did not show any pus formation throughout the treatment period. In the Planimetry assessment, wounds treated with polyherbal formulation showed a good score at the end of the treatment. Results showed that polyherbal formulation of Vaccinium macrocarpon and Cissampelos pareira extracts have good wound healing activity.

Wound healing activity, Vaccinium macrocarpon, Cissampelos pareira, Polyherbal gel, Escherichia coli

"Wound healing activity of polyherbal (gel) formulation of Cissampelos pareira and Vaccinium macrocarpon extracts", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 2, page no.b365-b377, February-2024, Available :http://www.jetir.org/papers/JETIR2402139.pdf

"Wound healing activity of polyherbal (gel) formulation of Cissampelos pareira and Vaccinium macrocarpon extracts", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 2, page no. ppb365-b377, February-2024, Available at : http://www.jetir.org/papers/JETIR2402139.pdf