• Swati Kothiyal
• R. C. Tiwari
• Bhawana Mittal
• Nidhi Belwal

Introduction: The present study was undertaken to explore the antioxidant properties of Maha Agad. Maha agad is one such formulation mentioned in classics which is used in acute toxicity conditions. The ingredients present in Maha agad are known for their rasayana karma which can be correlated with the antioxidant activity of the modern era. The primary objective of this study was to evaluate the antioxidant effect of Maha agad. Materials and methods: In this study, free radical scavenging activity, total phenolic content, total flavonoid content, ferric reducing power assay of ethanol and aqueous extracts of Maha Agad were measured spectroscopically. Free radical scavenging activity was determined according to the elimination of DPPH radicals, total phenol content was determined by the Folin–Ciocalteu method, total flavonoid content was determined by aluminum chloride colorimetric method, ferric reducing power assay was determined by using ascorbic acid as standard. Results: Both ethanol and aqueous extracts of Maha Agad had radical scavenging effects at all concentrations. The percentage inhibition of 200mg of ethanol extract exhibited the highest DPPH radical scavenging activity of 85% followed by aqueous extract of 81%. For ethanol extract, the Ic50 value ranged from 20 to 40 mg, whereas for aqueous extract, it was 40 to 80 mg. TPC values varies from 7.9 to 8.1mgGAE/g in ethanol extract and 7.8 to 7.9 mg GAE/g in aqueous extract. TFC values ranged from 13.57 to 13.79 mg QE/g in ethanol extract and 13.61 to 13.92 mg QE/g in aqueous extract. The range of FRAP values in ethanol extract was 3.3-26.7 μ mol Fe (II)/g, whereas in aqueous extract it was 8.4-20.5 μ mol Fe (II)/g. Conclusion: Results clearly showed that both the extracts of Maha Agad have showed good antioxidant activity. Ethanol extract of Maha Agad had strong DPPH radical scavenging, FRAP activity, and also have higher phenolic content whereas aqueous extract had high flavonoid content. As a result, Maha Agad can be used in pharmaceutical products as a source of natural antioxidants.

Antioxidant, Ethanol and Aqueous extract, Maha Agad, DPPH, Phenolic content, Flavonoid content, FRAP, Rasayana.

"Free radical scavenging activity, total phenolic content, total flavonoid content and ferric reducing antioxidant power assay of ‘Maha Agad’- A research article", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 2, page no.b458-b466, February-2024, Available :http://www.jetir.org/papers/JETIR2402152.pdf

"Free radical scavenging activity, total phenolic content, total flavonoid content and ferric reducing antioxidant power assay of ‘Maha Agad’- A research article", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 2, page no. ppb458-b466, February-2024, Available at : http://www.jetir.org/papers/JETIR2402152.pdf