• Sravani Gudikandula
• Navaneetha Nambigari

A ruthenium (II)polypyridyl complex of the type [Ru(NN)2L] where NN=phen,1,10 Phenanthroline and Intercalator ligand L=TMPIP=2-(3,4,5-Trimethoxy-phenyl)-H-imidazole[4,5-f][1,10]phenanthroline were synthesized and characterized by spectroscopic techniques like IR,1H NMR,13C NMR, Mass,UV and Elemental analysis. The DNA binding affinity of metal complexes were investigated by using Biophysical methods-Electronic absorption titration, Fluorescence emission titration and viscosity studies. The absorption and fluorescence binding constant (Kb) of complex with CT DNA is 1.5x105 and 2.1 x106 M-1 respectively. The data is comparable with the standard DNA metallointercalator ([Ru(bpy)2(dppz)]2+) due to the added planarity of the phenanthroline ligand and also the viscosity study reveal an intercalative mode of binding. In photocleavage studies the complexes successfully cleaved the pBR322 DNA. MTT assay of the complex reveal good anticancer activity against MCF-7 Cell line is IC50 = 17.42±0.29µg/ml indicating a correlation with DNA binding affinity.

Polypyridyl, Biophysical, DNA binding, photocleavage, anticancer activity.

"Correlation of Anti-cancer activity and DNA binding affinity of novel Ru(II) complex-Biophysical study", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 2, page no.c191-c201, February-2024, Available :http://www.jetir.org/papers/JETIR2402225.pdf

"Correlation of Anti-cancer activity and DNA binding affinity of novel Ru(II) complex-Biophysical study", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 2, page no. ppc191-c201, February-2024, Available at : http://www.jetir.org/papers/JETIR2402225.pdf