• JABEEN FARHANA
• Dr. G. Tulja Rani
• S.Yousif Ahmed

ABSTRACT In the present work, a simple, precise, accurate and cost effective extractive RP-HPLC method has been developed for the simultaneous estimation of the Acebrophylline and N-Acetylcysteine in Tablet dosage form. Chromatogram was run through Ascentis C18 150 x 4.6 mm, 5. Mobile phase containing Buffer OPA: Acetonitrile taken in the ratio 40:60 was pumped through column at a flow rate of 1.0ml/min. Buffer used in this method was OPA. Temperature was maintained at 30°C. Optimized wavelength selected was 225nm. Retention time of Acebrophylline and N-Acetylcysteine were found to be 2.855min and 2.280. %RSD of the Acebrophylline and N-Acetylcysteine were and found to be 0.7 respectively. %Recovery was obtained as 99.13% and 100.72% for Acebrophylline and N-Acetylcysteine respectively. LOD, LOQ values obtained from regression equations of Acebrophylline and N-Acetylcysteine were 0.10, 0.30 and 0.28, 0.86 respectively. Regression equation of Acebrophylline is y = 45305x + 13941 and y = 33545x + 5956.8 of N-Acetylcysteine. Retention times were decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Acebrophylline, N-Acetylcysteine, RP-HPLC

"DEVELOPMENT AND VALIDATION OF RPHPLC METHOD FOR QUANTIFICATION OF N-ACETYLCYSTIENE AND ACEBROPHYLLINE IN BULK AND ITS PHARMACEUTICAL FORMULATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 2, page no.c398-c410, February-2024, Available :http://www.jetir.org/papers/JETIR2402247.pdf

"DEVELOPMENT AND VALIDATION OF RPHPLC METHOD FOR QUANTIFICATION OF N-ACETYLCYSTIENE AND ACEBROPHYLLINE IN BULK AND ITS PHARMACEUTICAL FORMULATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 2, page no. ppc398-c410, February-2024, Available at : http://www.jetir.org/papers/JETIR2402247.pdf