• Dr.Mitesh A Bhatt
• Dr.Akash Shah
• Dr.Sandip Shah
• Dr.Bhavini Shah
• Mrs.Ankita Murnal

This article describes the simultaneous determination of immunosuppressants drugs in whole blood by tandem mass spectrometry. Here, a convenient ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method was develop and validate for the simultaneously determination of four compounds in whole blood. The whole blood samples, 50μL for each, extracted using a simple protein precipitation extraction method. The chromatographic separation was achieved on an Ultisil XB-CN column (50 mm × 2.1 mm, 3µm) at 40℃ by a gradient elution within 2.8min. The mobile phase was a mixture of methanol (phase A) and 5mM ammonium acetate with 0.1% glacial acetic acid (phase B) with a total flow rate of 0.4mL/min. The positive-ionization mode with multiple reaction monitoring was used for detection. The sensitivity was good with no carry-over detected, and the lower limit of quantification range. All standard curves showed favorable linearities with r2 > 0.99. The method has been successfully verified using authentic case samples that had previously been quantified using different methods. The assay is suitable for clinical utilization and management of patients on these medications

Immunosuppressants; Therapeutic drug monitoring; UPLC-MS/MS; human whole blood

"“ANALYTICAL TECHNOLOGY: LC-MS/MS FOR THERAPEUTIC IMMUNOSUPPRESSANTS DRUGS MONITORING IN WHOLE BLOOD.”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 3, page no.a460-a467, March-2024, Available :http://www.jetir.org/papers/JETIR2403059.pdf

"“ANALYTICAL TECHNOLOGY: LC-MS/MS FOR THERAPEUTIC IMMUNOSUPPRESSANTS DRUGS MONITORING IN WHOLE BLOOD.”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 3, page no. ppa460-a467, March-2024, Available at : http://www.jetir.org/papers/JETIR2403059.pdf