• Ms Pooja Mohan Deshmukhe
• Adarsh sawant
• Rutuja muskawad
• Atharva nikam
• Digvijay Patil

Azadirachta indica (Neem) hydroalcoholic extract was studied for its antifungal activity. The fresh matured Azadirachta indica leaves were collected, dried, powdered, extracted by using solvent (hydro-alcohol 50-50%) and used for further analysis. The result of qualitative phytochemical analysis proved that hydroalcoholic Azadirachta indica extract contains Secondary metabolites like alkaloids, flavonoids, tannins, saponins, glycosides. Further separation was carried out by column chromatography. Eluents were collected and RF value was calculated (0.28 cm). Preparative Thin layer chromatography was done by using mobile phase chloroform: ethyl acetate: formic acid (6:5:2). In vitro study was performed to investigate its antifungal effectiveness against the fungus candida albicans. Nimbin and azadirachtin are the two main chemical constituents responsible for activity. ZOI was found to be 22mm of neem extract while standard drug was found to be 25mm respectively. FT-IR study was carried out.

Azadirachta indica, Antifungal, Hydroalcohol, Chromatography.

"AZADIRACHTA INDICA AS A PROMISING TARGET RESPONSIBLE FOR ANTIFUNGAL ACTIVITY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 3, page no.d250-d266, March-2024, Available :http://www.jetir.org/papers/JETIR2403330.pdf

"AZADIRACHTA INDICA AS A PROMISING TARGET RESPONSIBLE FOR ANTIFUNGAL ACTIVITY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 3, page no. ppd250-d266, March-2024, Available at : http://www.jetir.org/papers/JETIR2403330.pdf