• B. Rajarajeshwari
• G. Renuga

The leather industry, which is one of the major contributors to pollution, has serious impacts on plants, animals and humans. The present study revealed the isolation of Pseudomonas putida from tannery effluent for its potency to detoxify toxic pollutants. Thus, the efficiency of hexavalent chrome reduction by the bacteria P. putida isolate was investigated by looking behind the plasmid-coded gene responsible for the degradation potential. Plasmid DNA was extracted from P. putida and then transformed into an XL-blue strain in order to check the role of plasmids in metal tolerance on LB plates containing 60 mM Cr. The metal tolerance and toxicity reduction coded by the plasmid gene confirmed by the PCR-coding gene might be around 9 kb and 4 kb, respectively. The coding region of the chrome reductase gene was amplified using appropriate primers. Based on the efficient Cr (VI) reducing ability of Pseudomonas putida, this bacterium has great potential for use in the detoxification of Cr (VI) in contaminated soil and water. Thus P.putida was recognized as a promising candidate to develop a safety strain for recombinant DNA. The present work focused on providing an attractive strategy for remediation of chromium from effluent, and then subsequently treated waste water could be considered for irrigation practices. Plant seed germination experiments were conducted to confirm its application for agricultural purposes. Results obtained by measuring shoot growth and root development, as well as the alpha-naphthalene oxidizing root activity of Oryza sativa seedlings, might explain the remediating efficiency of molecularly designed Pseudomonas putida, which plays a key role in the remediation of tannery effluent.

Alpha naphthalene assay, Chromate reductase, PCR, Plasmid, Oryza sativa.

"Molecular Design Of Pseudomonas putida For Remediation of Hexavalent Chromium In Tannery Effluent ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 3, page no.h443-h448, March-2024, Available :http://www.jetir.org/papers/JETIR2403759.pdf

"Molecular Design Of Pseudomonas putida For Remediation of Hexavalent Chromium In Tannery Effluent ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 3, page no. pph443-h448, March-2024, Available at : http://www.jetir.org/papers/JETIR2403759.pdf