• Ms.Tabassum Fatma
• Mr.Aman Abrar
• Mrs.Akanksha Awasthi
• Dharmendra Singh Gusain
• Mr.Rahul Verma

A monolayer slide preparation technique called liquid-based cytology (LBC) has surpassed traditional Pap smears due to enhanced fixation, a reduction in obscuring factors, and consistent cell transfer. Unlike conventional smears, in which the sample is smeared onto the glass slide and fixed separately, LBC involves collecting samples by fully submerging the sampling instrument into the company vial holding preservative solution. This preserves and fixes the cells concurrently. ThinPrep and SurePath are the two main liquid-based preparation techniques now in use. These two procedures have different cell harvesting principles, yet they create identical preparations. SurePath operates on the principle of density gradient sedimentation. A sample is vortexed and strained to break up mucus and big cell groupings, and then it is centrifuged using a density gradient. Staining with the PrepStain tool comes next. The SurePath method was approved by the FDA in 1999 and is used by Government Medical College and Hospital in Nagpur, India. Our organization uses the Rovers Cervex-Brush to gather cells from the transformation zone. The SurePath concept and the lab's fully automated cervicovaginal specimen processing method are covered in this chapter.

: Liquid-based cytology, SurePath, ThinPrep, Cervicovaginal, Cerves Brush Monolayer preparation, FDA, Harvesting

"LBC/HPV: Cervical cancer screening in the general Population-A Systematic Review", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 4, page no.a220-a231, April-2024, Available :http://www.jetir.org/papers/JETIR2404032.pdf

"LBC/HPV: Cervical cancer screening in the general Population-A Systematic Review", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 4, page no. ppa220-a231, April-2024, Available at : http://www.jetir.org/papers/JETIR2404032.pdf