• shruti pandey
• Ankurkumar Patel
• Murtaza Hajoori

DNA extraction from prokaryotic cells is a fundamental step in molecular biology and biotechnology research. It is challenging to achieve high-quality DNA yields with minimal contamination. This review emphasis on a comprehensive design approach for optimizing the DNA extraction procedure from prokaryotic cells. The methodology involves systematically assessing and fine-tuning various extraction parameters, including the choice of cell lysis method, selection of DNA purification techniques, optimization of reaction conditions (pH, temperature, incubation time), and integration of suitable controls to minimize experimental variability. The design approach emphasizes the importance of cell wall composition, DNA-binding properties, and enzymatic activity specific to prokaryotic cells. Additionally, advanced techniques such as bead beating, enzymatic digestion, and magnetic bead-based purification enhances DNA extraction efficiency and purity. The framework provides researchers with a systematic methodology to optimize DNA extraction protocols tailored to the unique characteristics of prokaryotic cells, enabling reliable and reproducible DNA isolation for downstream applications in genomics, metagenomics, and microbial ecology.

Extracted DNA, Optimization, Purification, High-quality DNA and Prokaryotes.

"Strategies for optimal DNA extraction from Prokaryotic Cells: A Comprehensive review", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 4, page no.b776-b783, April-2024, Available :http://www.jetir.org/papers/JETIR2404188.pdf

"Strategies for optimal DNA extraction from Prokaryotic Cells: A Comprehensive review", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 4, page no. ppb776-b783, April-2024, Available at : http://www.jetir.org/papers/JETIR2404188.pdf