• Ali Arkan Majhool
• Sultan Kareem Sultan
• Mohammed S.I.Al.Basha
• Ali M.Rayshan
• Hiba Mahdi Mahmood

In this study, the potential of rifampin production and characterize actinobacterial secondary metabolites using High-performance liquid chromatography (HPLC) analysis. A total of 9 samples were collected from different depths of Desert soils in Al-Anbar Governorate, Iraq, employing the spread plate technique on various agar media. Through 16s rRNA analysis, GenBank, and phylogenetic analysis, three rare actinobacterial strains were identified. To compare with crude extracts, rifampin was used as a standard for qualitative and quantitative HPLC analysis, based on rotation time. Following secondary metabolite extraction, HPLC analysis was conducted to characterize different extracts. The HPLC pattern analysis revealed that isolates AR7, AR21, and AR42 exhibited potential as rifampin producers. Additionally, secondary screening of crude extracts against six pathogenic microbes demonstrated varying degrees of inhibition zones. These findings underscore the significance of actinobacterial isolates in producing antibiotic compounds. Previous studies have established that actinobacteria harbor numerous compounds with antimicrobial, analgesic, anti-inflammatory, and antioxidant properties.

Keywords: Desert soil, Actinobacterial, Antimicrobial activity, Phylogenetic analysis, Metabolite extraction

"HPLC Photodiode Array Analysis of Rifampin in Actinobacterial Secondary Metabolites", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 4, page no.d108-d112, April-2024, Available :http://www.jetir.org/papers/JETIR2404316.pdf

"HPLC Photodiode Array Analysis of Rifampin in Actinobacterial Secondary Metabolites", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 4, page no. ppd108-d112, April-2024, Available at : http://www.jetir.org/papers/JETIR2404316.pdf