• Prof. Supriya S Bhosale
• Amruta A Ghogare
• Pratiksha V Gaikwad

Development and Validation of a High-Performance Liquid Chromatographic Analytical Procedure for Determining Levofloxacin in a Bulk and Marketed Dosage Formis described in this paper. The Separation was made with a C18 Symmetry (4.6 X 150mm,5μm) Column at ambient temperature, with isocratic mode and mobile phasePhosphate Buffer pH 2.8 : Acetonitrile 35:65 v/v. Eluent was monitored at 284nm and the flow rate was 1.0ml/min. LevofloxacinwaseffectivelySeparatedwithretentiontime(RT)of3.661minand5.116min respectively, within the selected chromatographic conditions. The Method was validated for AnalyticalParameters: Specificity, Linearity, Precision, Accuracyand Limits ofDetection and Quantitation. The Calibration curves were linear in the concentration range of 10 µg/ml for Levofloxacin and the Regression was found to be 0.995 respectively (NMT 0.999) for Levofloxacin. The % recovery for 50%, 100% and 150% accuracy level of Levofloxacin was found to be within the range of 99.3-100.3% respectively for Levofloxacin. This Analytical Procedure is applicable for the Quality Control of Drug Formulations

Levofloxacin, Stationary Phase, Mobile Phase, HPLC, Validation, Phosphate Buffer, Acetonitrile

"Development and Validation of HPLC Method for the Estimation of Levofloxacin in Bulk and Marketed Dosage form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 4, page no.l326-l338, April-2024, Available :http://www.jetir.org/papers/JETIR2404B45.pdf

"Development and Validation of HPLC Method for the Estimation of Levofloxacin in Bulk and Marketed Dosage form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 4, page no. ppl326-l338, April-2024, Available at : http://www.jetir.org/papers/JETIR2404B45.pdf