• MORUPOJU SUSHMA
• B.Naga jyothi
• K.Sri chaya reddy

A new RP-HPLC for the simultaneous estimation of Tipranavir was developed by optimizing the chromatographic conditions using Inertsil ODS C18 column (4.6×150mm) 5µ and the flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) methanol: phosphate buffer (KH2PO4and K2HPO4) phosphate pH 3 (pH was adjusted with orthophosphoricacid),detection wavelength was 265nm.the instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empower-software version-2. The retention times were found to be 1.988 mins .The % purity of Tipranavir was found to be 99.24% and. The system suitability parameters for Tipranavir such as theoretical plates and tailing factor were found to be2689, 1.4 and the resolution was found to be 8.86. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Tipranavir was found in concentration range of 10µg-60µg and correlation coefficient (r2) was found to be 0.999, recovery was found to be 99.56%,%RSD for repeatability was 0.5, % RSD for intermediate precision was 0.6 respectively. The precision study was precision, robustness and repeatabilty.LOD value was 1.97 and LOQ value was 1.97. Hence the suggested RP-HPLC method can be used for routine analysis of Tipranavir in API and Pharmaceutical dosage form.

tipranavir,RP-HPLC, correlation coefficient.

"DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR THE DETERMINATION OF TIPRANAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 5, page no.d709-d716, May-2024, Available :http://www.jetir.org/papers/JETIR2405380.pdf

"DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR THE DETERMINATION OF TIPRANAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 5, page no. ppd709-d716, May-2024, Available at : http://www.jetir.org/papers/JETIR2405380.pdf