• Sushil Vasanta kumbhare
• Yaminee J. Sathawane
• Dr. Krishna Gupta
• Dr. Milind J. Umekar

A Sensitive, selective, rapid, precise, and economical Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method. Tetrabenazine pharmaceutical dosage form. In standard absorptivity value, determination was carried out at absorption maxima of 254 nm using methanol as a solvent where as in used. HPLC method was carried out on a column XBridgeTM C183.5µm with a mobile phase consisting of Methanol: Acetonitrile in the ratio of (50:50 v/v) at a flow rate of 1.0ml/min. Detection was carried out at 284 nm with help of photodiode array (PDA) detector. The retention time of Tetrabenazine was 3.9 min. All the determination in High Performance Liquid Chromatographic (HPLC) methods were carried out in linearity range of 2-10 µg/ml. UV- Spectrophotometric methods were checked for accuracy and precision were as High-Performance Liquid Chromatographic (HPLC) method was validated as per international conference on Harmonization (ICH) guidelines. The proposed methods can be used for the routine estimation of the drug in bulk and pharmaceutical dosage form.

"Development and validation of stability indicating RP-HPLC method for Tetrabenazine from its pharmaceutical dosage form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 5, page no.h318-h327, May-2024, Available :http://www.jetir.org/papers/JETIR2405739.pdf

"Development and validation of stability indicating RP-HPLC method for Tetrabenazine from its pharmaceutical dosage form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 5, page no. pph318-h327, May-2024, Available at : http://www.jetir.org/papers/JETIR2405739.pdf