• Suraj Narayan Lohani
• Dr. Ajay Kumar Tilawat
• Shreya Sharma

This study provides in-depth insight into yield and quality assessment through a detailed analysis of saliva DNA extraction technology. Saliva sampling is a non-invasive and painless method, and its importance is increasing in various fields such as genetic research, disease diagnosis, and forensic medicine. This allows DNA samples to be collected without burden on patients or subjects, which can greatly improve the efficiency of research and diagnosis. This study compares and analyzes various methods for extracting DNA from saliva and evaluates in detail the impact of each method on DNA yield and quality. This includes a variety of cutting-edge technologies, including traditional precipitation and column-based extraction methods, automated extraction systems, and microfluidics technology. Through this comparative analysis, this study presents criteria to help select the most appropriate DNA extraction methodology for the characteristics of specific saliva samples. This study also investigates in detail the impact of the diversity and complexity of saliva samples on the DNA extraction process and explores effective strategies to overcome these challenges. The composition of saliva samples can vary from person to person, which can affect the yield and quality during the extraction process. Based on this understanding, this study proposes a method to maximize the efficiency of the DNA extraction process and improve the quality of the results. These research results aim to make an important contribution to the field of biological sampling by exploring the current status and future development potential of DNA extraction technology through saliva. In addition, the results of this study are expected to provide valuable information to researchers working in the fields of genetic research, disease diagnosis, and forensic medicine, contributing to the development of these fields.

Salivary DNA extraction, non-invasive sampling, DNA yield, DNA quality assessment, extraction technique, genetic research, disease diagnosis, forensics, sample diversity, extraction methodology selection criteria

"Insights into Saliva DNA Extraction: Techniques, Yield, and Quality Assessment", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 5, page no.k761-k773, May-2024, Available :http://www.jetir.org/papers/JETIR2405A94.pdf

"Insights into Saliva DNA Extraction: Techniques, Yield, and Quality Assessment", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 5, page no. ppk761-k773, May-2024, Available at : http://www.jetir.org/papers/JETIR2405A94.pdf