• RAVI RANJAN
• KAUSHAL KUMAR MAHATO

Since Friedrich Miescher's pioneering DNA extraction in 1869, scientists have continually refined methods to make them safer, more efficient, and environmentally friendly. Traditional liquid-liquid extraction, reliant on hazardous reagents like phenol-chloroform, has given way to physical extraction techniques, offering simplicity and safety. These innovations, such as magnetic bead and cellulose-based filter paper extraction, have revolutionized DNA handling. In the era of gene editing and personalized medicine, the demand for precise, high-yield DNA isolation methods has surged. Consequently, this review explores the transition from solvent-based to physical extraction methods, each presenting distinct advantages and limitations. This protocol emphasizes a novel approach: employing in-house prepared reagents for DNA isolation. By utilizing locally sourced materials, researchers can reduce costs and dependency on commercial products. Additionally, this approach promotes sustainability and flexibility, as reagents can be tailored to specific research needs. In summary, this review underscores the evolution of DNA extraction techniques, highlighting the shift towards safer, more efficient methods. By embracing innovative strategies like in-house reagent preparation, researchers can meet the growing demand for reliable DNA isolation while minimizing environmental impact and resource dependency 

DNA, DNA Extraction Methods, DNA Isolation, Purification

"A REVIEW ON DNA ISOLATION METHOD", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 5, page no.o368-o370, May-2024, Available :http://www.jetir.org/papers/JETIR2405F39.pdf

"A REVIEW ON DNA ISOLATION METHOD", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 5, page no. ppo368-o370, May-2024, Available at : http://www.jetir.org/papers/JETIR2405F39.pdf