• Uma Kumari (Third Author)
• Shikha Shrivastava (First Author)
• Neeta Shweta Kerketta (Second Author)
• Uma Kumari
• Vinay Kumar Oraon

Pseudomonas aeruginosa is a motile, gram-negative bacillus that possesses flagella. One of this microorganism's virulence factors is biofilm development. It can infect adults as well as newborns or young children with nosocomial infections. It initiates the production of its biofilm by signalling the other bacteria to join it through a process called quorum sensing. It led to serious infections in patients with weakened immune systems.It produces multidrug-resistant chronic infections as a result of biofilm formation. Encased in an extracellular matrix consisting of protein, extracellular DNA, and exopolysaccharides, biofilms are intricate microbial cells that provide protection against pathogenic agents.Numerous investigations have come to the conclusion that P. aeruginosa biofilm development is influenced by pH. In terms of pH, P. aeruginosa (5.3 and 9) resembles life in the human host (7.5). the initial Pseudomonas sp. report. Three distinct temperatures were chosen in order to determine the production of biofilms at pH values of pH-5, pH-3, and pH-9. Since pH5 is typically used for growth in P. aeruginosa biofilm investigations, it's possible that these unexplored factors to biofilm integrity were previously disregarded. Our research showed that changes in pH can have significant effects on the development of biofilms and emphasise the significance of environmental factors. At pH-5, which is the ideal pH, particular changes in biofilm physiology allowed for normal growth, but at pH-3, no such biofilm forms.

(Keywords: Biofilm, Quorum Sensing, Virulence, Pseudomonas aeruginosa, Adhesion)

"EFFECT OF pH ON THE PSEUDOMONAS AERUGINOSA BIOFILM FORMATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 6, page no.j710-j716, June-2024, Available :http://www.jetir.org/papers/JETIR2406975.pdf

"EFFECT OF pH ON THE PSEUDOMONAS AERUGINOSA BIOFILM FORMATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 6, page no. ppj710-j716, June-2024, Available at : http://www.jetir.org/papers/JETIR2406975.pdf