• Dr. AMIT M. PADELKAR
• ANIL NISHAD
• Dr. GAYATRI BARABDE

A new high-performance liquid chromatography (HPLC) method for the quantitation of Rafoxanide has been developed and validated. Rafoxanide was subjected to thermal decomposition at 120, 130, 140, 150, 160,170°C. Samples were withdrawn at intervals of 7, 14, 21 and 28 days and analyzed for remaining drug content by HPLC assay method. The samples were eluted on a Cosmosil C18 column (250 mm x 4.6 mm, 5.0 μm) at 40°C, with a mobile phase of 3% of glacial acetic acid in Methanol : Water :: 95 : 5, (v/v). The flow rate was 1.5 mL/min, injection volume :20µL and detection by PDA detector with detection wavelength at 284 nm. Rafoxanide shows thermal-degradation after exposure to high temperature. The shelf life of Rafoxanide was determined by accelerated stability studies on the basis of first order degradation kinetics. The reaction rate constants, activation energy and half life were also calculated using Arrhenius equation and plots. The shelf life of Rafoxanide was found to be approximately (t90 ) 5.75 years. Amounts of Rafoxanide in all samples were determined by HPLC and the results were compared with those from Infra red spectroscopy and X-ray diffraction technology for qualitative comparison.

Rafoxanide, Arrhenius equation, accelerated stability testing, shelf-life, HPLC.

"DETERMINATION OF PRODUCT SHELF LIFE AND EVALUATION OF KINETIC PARAMETERS OF THERMAL DECOMPOSITION OF RAFOXANIDE IN SOLID STATE BY HPLC.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 7, page no.c349-c357, July-2024, Available :http://www.jetir.org/papers/JETIR2407244.pdf

"DETERMINATION OF PRODUCT SHELF LIFE AND EVALUATION OF KINETIC PARAMETERS OF THERMAL DECOMPOSITION OF RAFOXANIDE IN SOLID STATE BY HPLC.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 7, page no. ppc349-c357, July-2024, Available at : http://www.jetir.org/papers/JETIR2407244.pdf