• Dhanashri Dhasade
• Smita Nayak
• Vijay Kumar Munipalli
• Bhushan Sonawane
• Vaidhun Bhaskar

A novel, selective, precise, and high performance thin layer chromatographic technique has been developed and validated for estimating Abemaciclib in tablet dosage form. TLC aluminium plates precoated with silica gel 60 F-254 were used as the stationary phase employed TLC Aluminium plates precoated with silica gel 60 F- 254 as the stationary phase. Linear ascending development with Toluene: acetone: methanol: ammonia in the ratio 8:2:2:0.1 (v/v) as the mobile phase. It was concluded at room temperature (25± 20C) phase was performed at room temperature (25 ± 2°C) in a twin trough glass chamber saturated with the mobile phase. Abemaciclib has compact bands (RF 0.42 ± 0.02). Scanning was done in absorbance mode at 298 nm. Linear regression analysis of calibration plots revealed a strong linear association between peak area and peak height (R2=0.9995 and 0.997) in the concentration range of 50-400 ng/band. The method was tested for specificity, precision, accuracy, robustness, detection and quantification limits. The detection and quantification limits were 9.75 and 29.70 ng/band, respectively. The approach achieved recovery rate of 100.28%. This approach can be used as a quality control method to check the purity of Abemaciclib in its tablet dosage form.

Abemaciclib, HPTLC, Method development, Validation.

"Novel HPTLC Method Development and Validation for Estimation of Abemaciclib in Tablet Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 7, page no.f348-f356, July-2024, Available :http://www.jetir.org/papers/JETIR2407549.pdf

"Novel HPTLC Method Development and Validation for Estimation of Abemaciclib in Tablet Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 7, page no. ppf348-f356, July-2024, Available at : http://www.jetir.org/papers/JETIR2407549.pdf