Abstract
The study investigates the performance of bio-adsorbents—specifically plantain stem fiber, banana stem fiber, and palm fruit fiber—in a packed bed unit for mitigating total petroleum hydrocarbons (TPH) from freshwater environments. The bio-adsorbents were prepared by drying the agricultural materials in sunlight and indoors, then grinding them into powder with particle sizes of 50μm, 150μm, and 200μm, mixed in equal proportions (1:1:1 ratio by mass). Each packed bed unit, connected in series, contained 1500g of this mixed adsorbent. The experimental setup maintained a constant flow rate of 0.2m^3/sec and a packed bed height of 27cm. The primary focus was on evaluating the influence of temperature on physicochemical properties, microbial activity, TPH removal efficiency, residence time, and remediation rates within the packed bed units. Microbial analysis identified predominant microbial strains (PSRTR, PFFRTR, BSRTR, etc.) associated with each bio-adsorbent type under varying temperature conditions. Results indicated higher microbial counts in units using room-dried plantain fiber compared to other configurations (PSRTR1 > PFFRTR1 > BSRTR1, etc.). Notably, room-dried plantain fiber exhibited superior performance across experimental runs (U1, U2, U3). Temperature significantly influenced the bio-adsorption process, impacting fluid penetration, diffusion rates (residence time), and consequently, TPH removal efficiency. Optimal contaminant removal was observed at 45°C, highlighting temperature's pivotal role in enhancing bio-adsorbent effectiveness. This research underscores the critical impact of temperature variations on bio-adsorbent performance in packed bed units during bioremediation processes. Specifically, it highlights the efficacy of plantain fiber in TPH removal when subjected to optimal temperature conditions. These findings contribute valuable insights into optimizing environmental remediation strategies, emphasizing the practical application of agricultural waste-derived bio-adsorbents in mitigating hydrocarbon pollutants from aquatic systems.