• Mohini Bagul
• Smita Nayak
• Bhaskar Vaidhun
• Bhushan Sonawane
• Vijay Kumar Munipalli

A novel, accurate, exact, sensitive and appropriate High Performance Thin Layer Chromatography (HPTLC) method was developed and validated for the determination and estimation of Pimobendan in pharmaceutical tablet dosage form. The chromatographic separation for Pimobendan was achieved on Silica gel 60 F254 HPTLC plate as the stationary phase using a standardized mobile phase composed of n-Hexane: Ethyl acetate: Ethanol: Glacial acetic acid (GAA) in the following ratio of 70:15:15:1%v/v/v/v. Identification and quantification of Pimobendan were done at 330nm. The system was found to produced sharp peak and well resolved discrete band at an appropriate Rf value i.e. 0.442. The performance of proposed method was validated with respect to linearity, recovery, precision, robustness, LOD and LOQ in accordance with ICH guidelines. The linearity for molecule was found between the range of 4-40ng/band with correlation coefficient 0.9991. The LOD (limit of detection) and LOQ (limit of quantification) were found to be 1.04 ng/band and 3.16 ng/band respectively. The current method is novel since there is no reported HPTLC method for the estimation of Pimobendan in pharmaceutical tablet dosage form and method can be used successfully for quality checks and routine analysis of Pimobendan.

Pimobendan, High Performance Thin Layer Chromatography, CAMAG Linomat 5, LOD, LOQ

"Development And Validation of Novel HPTLC Method for The Estimation of Pimobendan In Tablet Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 7, page no.g494-g502, July-2024, Available :http://www.jetir.org/papers/JETIR2407657.pdf

"Development And Validation of Novel HPTLC Method for The Estimation of Pimobendan In Tablet Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 7, page no. ppg494-g502, July-2024, Available at : http://www.jetir.org/papers/JETIR2407657.pdf