• Akshay Rajput
• Dr. Preeti Kulkarni
• Dr. Vijay Kumar Munipalli
• V.H. Bhaskar
• Hermant Magar

The estimate of acalabrutinib in pharmaceutical tablet dosage form was accomplished through the development and validation of a unique, accurate, exact, sensitive, and appropriate High Performance Thin Layer Chromatography (HPTLC) method. The chromatographic separation of acalabrutinib was accomplished on a Silica gel 60 F254 HPTLC plate as the stationary phase. The mobile phase consisted of a 75: 15: 5: 1% v/v/v/v mixture of toluene, ethanol, ethyl acetate, and glacial acetic acid (GAA). At 230 nanometres, acalabrutinib was detected and quantified. It was discovered that the system produced a sharp peak and a well-resolved discrete band at an appropriate Rf value of 0.26. The suggested method's performance was verified in accordance with ICH requirements for robustness, linearity, accuracy, precision, LOD, and LOQ. A correlation coefficient of 0.9998 was discovered to indicate the linearity of the molecule within the 10-80ng/band range. It was discovered that the limits of detection and quantification were, respectively, 0.004 µg/ml and 0.14 ng/band. Since there was no prior HPTLC method for estimating acalabrutinib in pharmaceutical tablet dose form, the current method was innovative. It was also effectively employed for regular analysis and quality checks of the drug.

"DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE ESTIMATION OF ACALABRUTINIB IN TABLET DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 9, page no.c533-c542, September-2024, Available :http://www.jetir.org/papers/JETIR2409259.pdf

"DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE ESTIMATION OF ACALABRUTINIB IN TABLET DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 9, page no. ppc533-c542, September-2024, Available at : http://www.jetir.org/papers/JETIR2409259.pdf