• Sajeeda begum
• Savitha
• Vamsi krishna bonagiri
• M. Laya
• K. Maheshwari

High-Performance liquid chromatography is a critical analytical technique widely used for separating, identifying, and quantifying components in a mixture. Despite its robustness, various issues can arise during HPLC operations, impacting the accuracy and reliability of results. Troubleshooting is needed to develop and maintain complex systems where the symptoms of a problem can have many possible causes. In this article we provide a comprehensive overview of typical HPLC problems and their potential causes, including solutions and preventive measure to enhance performance and ensure accurate analysis.

Troubleshooting, HPLC, Resolution, Retention Factor, Selectivity, Column Back Pressure, Retention time variation, Incorrect peak shapes.

"HPLC TROUBLESHOOTING: A REVIEW", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 9, page no.d507-d519, September-2024, Available :http://www.jetir.org/papers/JETIR2409359.pdf

"HPLC TROUBLESHOOTING: A REVIEW", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 9, page no. ppd507-d519, September-2024, Available at : http://www.jetir.org/papers/JETIR2409359.pdf