• Neha Ailani
• Dr Bela Nabar

Abstract: Burn wound infections are of global healthcare concern due to the increased incidence of emergence of antibiotic-resistant strains in the burn wound niche. In lieu of this critical challenge, current research work explores a nature-inspired arsenal in the battle against these resistant species causing burn wound infections. Plant-based products to control infection and help in faster wound healing. The study investigates the anti-bacterial properties of alcoholic and aqueous extracts prepared in consortia using Ashwagandha (Withania somnifera), Tulsi (Ocimum sanctum or Holy Basil) and Pot marigold (Calendula officinalis) at its effective Inhibitory Concentration. Inhibition of bacteria causing burn wound infection was observed at 1.5 % (15 mg/ml) concentration of polyherbal consortia. The consortia were then studied to assess their cytotoxicity and wound healing potential in vitro. The Percentage cellular viability and proliferation of 3T3 murine fibroblast cell lines by the consortium was studied at the concentration range of 25 µg/mL to 500 µg/mL. Percentage viability assay performed by colorimetric MTT assay showed negligible effect on viability & proliferation of fibroblast cells even at higher concentration of 500 µg/mL. Thus, to conclude, the consortium of extract prepared using Ashwagandha, Tulsi, and Calendula exhibits promising antibacterial activity with no adverse effects on fibroblast viability and migration, emphasizing its therapeutic potential in burn wound treatment.

Burn wound infections, Drug resistance, polyherbal extracts, in vitro cytotoxicity assessment, Scratch assay, MIC

"STUDY THE EFFECT OF CONSORTIUM OF WITHANIA SOMNIFERA, OCIMUM SANCTUM AND CALENDULA OFFICINALIS IN MANAGEMENT OF BURN WOUND INFECTIONS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 10, page no.e222-e226, October-2024, Available :http://www.jetir.org/papers/JETIR2410423.pdf

"STUDY THE EFFECT OF CONSORTIUM OF WITHANIA SOMNIFERA, OCIMUM SANCTUM AND CALENDULA OFFICINALIS IN MANAGEMENT OF BURN WOUND INFECTIONS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 10, page no. ppe222-e226, October-2024, Available at : http://www.jetir.org/papers/JETIR2410423.pdf