• Ranjeet kannaujiya
• Mr.Manish Sharma
• Dr.Sukhwant Singh
• Dr.Jitendra Banweer

Abstract This study focused on the analytical method development and validation for the determination of flavonoids in Artocarpus heterophyllus using reverse-phase high-performance liquid chromatography (RP-HPLC). The organoleptic evaluation confirmed the plant's dark green color and foliaceous texture. Aqueous extraction yielded 10.9% of the extract, which was subjected to phytochemical screening, revealing the presence of alkaloids, flavonoids, phenols, proteins, carbohydrates, and saponins. TLC analysis indicated the presence of multiple flavonoid compounds, with Rf values suggesting the presence of quercetin. Quantitative analysis demonstrated total phenolic and flavonoid contents of 0.221 mg/100 mg and 0.763 mg/100 mg, respectively. Method validation showed excellent linearity (r2 = 0.999), recovery rates near 100%, and high precision and robustness. The mean quercetin content in the extract was found to be 0.810%. These findings highlight the potential of Artocarpus heterophyllus as a source of bioactive flavonoids, supporting its application in nutraceuticals.

Artocarpus heterophyllus, flavonoids, RP-HPLC, phytochemical screening, TLC analysis, method validation, bioactive compounds, quercetin, aqueous extract

"“ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FORDETERMINATION OF FLAVONOIDS BY RP-HPLC” ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 11, page no.e38-e45, November-2024, Available :http://www.jetir.org/papers/JETIR2411406.pdf

"“ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FORDETERMINATION OF FLAVONOIDS BY RP-HPLC” ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 11, page no. ppe38-e45, November-2024, Available at : http://www.jetir.org/papers/JETIR2411406.pdf