• E. Naga Deepthi
• P. Tejaswani

The hyphenated techniques are the combination or the coupling of the different analytical techniques such as Chromatographic (separation) techniques are combined with Spectroscopic (detection) techniques. Here the separated components of the mixture from Chromatographic technique will enter into the Spectroscopic (detection) technique through an interphase. Hence the compounds can be separated and detected simultaneously. Hyphenated techniques couple two (or more) methods such as GC-MS, LC-MS, LC-NMR, and CE-MS to solve more complex analytical problems. This technology offers faster analysis times, higher automation, better throughput, better reproducibility, and less pollution. Hyphenated techniques play a vital role in various applications in the pharmacy especially in the analysis of biomaterials, chemical fingerprinting, analytical chemistry, chemotaxonomy, carotenoids, essential oil and volatile components, alkaloids, coumarins, saponins. This article discusses the latest advances in various techniques such as GC-MS, LC-MS, LC-NMR, and CE-MS, these advanced techniques have its greatest role in enhancing the production of pure compounds.

Hyphenated technique, GC-MS, Analytical chemistry, LC-NMR, Dereplication, LC-IR, Reproducibility, chromatography, Spectroscopy, Automation, Analytical method.

"Synergistic Hyphenation: Merging Analytical Techniques For Precision", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 11, page no.g317-g330, November-2024, Available :http://www.jetir.org/papers/JETIR2411629.pdf

"Synergistic Hyphenation: Merging Analytical Techniques For Precision", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 11, page no. ppg317-g330, November-2024, Available at : http://www.jetir.org/papers/JETIR2411629.pdf