• Kondappagari Srikanth Reddy
• Rajasekaran.s

A simple, rapid, precise and reliable RP-HPLC method was developed for Simultaneous estimation of Repaglinide and Voglibose in pharmaceutical dosage form. The method is based of precolumn derivatization of Voglibose with Acetonitrile with Water, Acetonitrile with Methanol it cannot directly estimated by UV detector in RP-HPLC method. Precolumn derivatization (PCD) conditions were optimized by evaluating the parameters such as concentration methanol and water, concentration and reaction time of derivatization. The chromatographic separation was achieved on Inertsil-ODS C18 (250mm x 4.6mm, 5μm) column and Methanol: water (50:50v/v) as a mobile phase at a flow rate of 1ml/min. Detection was carried out at 252nm. The Retention time of Repaglinide-4.714min and Voglibose-6.691minute. The method has been validated for linearity, accuracy and precision. Linearity range observed 20-80 ppm. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Repaglinide and Voglibose in its Tablet. The drug was subjected to stress condition of hydrolysis, oxidation, photolysis and proposed method was successfully applied for the simultaneous estimation of the drugs in commercial combined dosage form.

"DEVELOPMENT AND VALIDATION OF STABILITY INDICATING CHROMATOGRAPHIC METHODS FOR ESTIMATION OF HYPOGLYCEMIC DRUGS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 12, page no.a969-a981, December-2024, Available :http://www.jetir.org/papers/JETIR2412087.pdf

"DEVELOPMENT AND VALIDATION OF STABILITY INDICATING CHROMATOGRAPHIC METHODS FOR ESTIMATION OF HYPOGLYCEMIC DRUGS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 12, page no. ppa969-a981, December-2024, Available at : http://www.jetir.org/papers/JETIR2412087.pdf