• Dr Prajwal C.R
• Dr Nisarga K.S
• Dr Anuradha K.N
• Dr Vishwanatha

Introduction-Cancer is a major reason people die around the world. Malignant melanoma is the most aggressive form of skin cancer, with a high mortality rate. More than 98% of the skin cancer are non-melanoma skin cancer (squamous cell cancer and basal cell cancer) the rest 1-2% are Melanoma skin cancer. One of the main problems in cancer treatment is gradual resistance mechanism. Recently herbal medicines are providing better management and preventive measure for cancer. The preliminary studies on a number of promising herbal drugs having anticancer potential. The main purpose of this study is to look into how protective Azadirachta indica A. Juss stem bark is in-vitro. Objectives- To Evaluate and Compare the effect of aqueous extract and hydro-alcoholic extract of stem bark of Nimba, Azadirachta indica A Linn on skin carcinoma in vitro. Methods and materials: Aqueous and hydro-alcoholic extract of Stem bark of Nimba (Azadirachta indica A. Juss) was evaluated for its anticancerous activity against A-431 skin cell lines by MTT -Assay. Nimba plant is attributed with various medicinal properties like Kushtaghna, Kandughna, Raktashodhaka, Vranaropana and Shophahara. Neem leaf extract have shown efficacy against a variety of human carcinoma cell lines and Animal models. Chemical constituents in both Extracts of leaf and Stem bark of Nimba are identical to each other. Result-There was a significant increase in percentage of cell death with increasing concentration showed significant result in both aqueous and hydro- alcoholic extract. Hydro alcoholic extract 89% cell death more effective than Aqueous extract. However standard drug Cisplatin showed 0.6% of cell death at 1000 μg /ml. Conclusion-Hydro-alcoholic extract of stem bark of Nimba (Azadirachta indica A Linn) is proven to have anti-cancerous activity, when tested on Skin cell line A-431 MTT assay.

Index Term- Skin cancer, Nimba, Aqueous and hydro-alcoholic extract, A-431 skin cell line, MTT -Assay

"SCREENING FOR ANTICANCEROUS ACTIVITY OF STEM BARK OF NIMBA (AZADIRACHTA INDICA A. JUSS.)-AN IN-VITRO STUDY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 12, page no.b873-b884, December-2024, Available :http://www.jetir.org/papers/JETIR2412185.pdf

"SCREENING FOR ANTICANCEROUS ACTIVITY OF STEM BARK OF NIMBA (AZADIRACHTA INDICA A. JUSS.)-AN IN-VITRO STUDY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 12, page no. ppb873-b884, December-2024, Available at : http://www.jetir.org/papers/JETIR2412185.pdf