• RITU LALIT JAIN
• AFIYA SHAIKH

In vitro techniques offer multiple advantages over traditional soil-based methods. Understanding In vitro spore germination and early gametophyte development can help optimize the growth and establishment of Pteris vittata for phytoremediation. The study aimed to investigate the development of early gametophytes in P. vittata and understand the morphology and development of the gametophyte in different growth media. To achieve this, spores were sown in sterile Petri dishes with different culture mediums. Knop's media, Knop's + Nitsch trace elements, and a control dish containing distilled water. The objective was to determine how different nutrient conditions affect the development of gametophytes. Weekly observation was carried out to monitor the germination of spores and the growth of the spatulate and coordinate gametophyte stages. The average spore germination rate ranged from 90% to 100%. Notably, the results indicated that the highest spore germination and the development of the spatulate and coordinate gametophyte stages occurred when the media was supplemented with Knops and Nitsch trace elements.

gametophyte, In vitro ,spatulate

"CHARACTERIZATIO OF THE DEVELOPMENT OF EARLY GAMETOPHYTES FROM SPORES OF PTERIS VITTATA .", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 12, page no.e462-e469, December-2024, Available :http://www.jetir.org/papers/JETIR2412451.pdf

"CHARACTERIZATIO OF THE DEVELOPMENT OF EARLY GAMETOPHYTES FROM SPORES OF PTERIS VITTATA .", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 12, page no. ppe462-e469, December-2024, Available at : http://www.jetir.org/papers/JETIR2412451.pdf