• Samkit
• Manoj Kumari More
• Kavita R Loksh

In the present work, the primary objective was to isolate flavonoid from the hydro-alcoholic extract of Alternanthera sissilis plant. The plant was powdered after drying and extracted with ethanol-water (70:30) after defatting with petroleum ether, to obtain dark brown sticky extract in 16.37% yield. The qualitative phytochemical screening suggested the presence of alkaloid, glyocoside, tannins, phenolics, flavonois and sterols in the extract. The amount of phenolic present in the hydro-alcoholic extract was found to be 109.18 GAE mg/g of extract.The amount of phenolic present in the hydro-alcoholic extract was found to be 76.27 QE mg/g of extract. The amount of phenolic present in the hydro-alcoholic extract was found to be 50.16 CE mg/g of extract. The hydro-alcoholic extract was analyzed by TLC for separation of components. The optimized solvent system was Toluene: Ethyl acetate: Acetic acid (4:1:0.2) and resulted in 6 components from the hydro-alcoholic extract. Active constituents were isolated from column chromatography with the mobile phase of Toluene: Ethyl acetate: Acetic acid (4:1:0.2) to obtain 18 fraction of 25 mL each. The fraction F3 was found to contain a single compound as visualized from TLC and was evaporated to obtain the solid product. This solid was recrystallized from propan-2-ol to obtain the pure isolated compound. The isolated compound was studied using FT-IR, mass and NMR spectral characterization to identify the compound. The isolated compound was proposed to have flavonoid structure and from the molecular weight it was proposed to be the flavonoid quercetin-O-glucoside.

"Isolation and characterization of flavonoid from Alternanthera sessilis", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 12, page no.e656-e664, December-2024, Available :http://www.jetir.org/papers/JETIR2412474.pdf

"Isolation and characterization of flavonoid from Alternanthera sessilis", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 12, page no. ppe656-e664, December-2024, Available at : http://www.jetir.org/papers/JETIR2412474.pdf